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Calpain 11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15673

Novus Biologicals, part of Bio-Techne
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NBP2-15673

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 11. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%).

Localization

Acrosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 11 Antibody

Western Blot: Calpain 11 Antibody [NBP2-15673]

Western Blot: Calpain 11 Antibody [NBP2-15673]

Western Blot: Calpain 11 Antibody [NBP2-15673] - Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Calpain 11 Antibody [NBP2-15673]

Immunocytochemistry/ Immunofluorescence: Calpain 11 Antibody [NBP2-15673]

Immunocytochemistry/Immunofluorescence: Calpain 11 Antibody [NBP2-15673] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Calpain 11 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Calpain 11 Antibody [NBP2-15673]

Immunohistochemistry-Paraffin: Calpain 11 Antibody [NBP2-15673]

Immunohistochemistry-Paraffin: Calpain 11 Antibody [NBP2-15673] - Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using antibody at 1:500 dilution.

Applications for Calpain 11 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpain 11

Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis. [provided by RefSeq]

Alternate Names

Calcium-activated neutral proteinase 11, calpain 11, calpain11, calpain-11, CANP 11, EC 3.4.22, EC 3.4.22.-, EC 3.4.22.52, EC 3.4.22.54

Gene Symbol

CAPN11

UniProt

Additional Calpain 11 Products

Product Documents for Calpain 11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Calpain 11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.