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Calpain 2 Antibody (17E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26467

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-26467-100ul
NBP3-26467-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 17E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Calpain 2 Antibody (17E9)

Immunogen

A synthesized peptide derived from Human Calpain 2 [UniProt P17655]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 2 Antibody (17E9)

Calpain 2 Antibody (17E9)

Western Blot: Calpain 2 Antibody (17E9) [NBP3-26467] -

Western Blot: Calpain 2 Antibody (17E9) [NBP3-26467] - Positive Western Blot detected in: U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate.
All lanes: Calpain 2 Antibody at 1: 500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 80, 72 kDa
Observed band size: 72-95 kDa

Applications for Calpain 2 Antibody (17E9)

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Calpain 2

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Alternate Names

Calcium-activated neutral proteinase 2, calpain 2, (m/II) large subunit, calpain 2, large [catalytic] subunit, calpain 2, large subunit, Calpain large polypeptide L2, Calpain M-type, calpain, large polypeptide L2, calpain-2 catalytic subunit, Calpain-2 large subunit, CANP 2, CANPL2CANP2, CANPml, EC 3.4.22, EC 3.4.22.53, FLJ39928, M-calpain, mCANP, millimolar-calpain

Gene Symbol

CAPN2

Additional Calpain 2 Products

Product Documents for Calpain 2 Antibody (17E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Calpain 2 Antibody (17E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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