Calpain 2 Antibody (OTI1F4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01814

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1F4

Concentration

0.75 mg/ml

View all Calpain 2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

79.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 2 Antibody (OTI1F4)

Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: Calpain 2 Antibody (OTI1F4) [NBP2-01814]

Flow Cytometry: Calpain 2 Antibody (OTI1F4) [NBP2-01814]

Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpain 2 antibody, and then analyzed by flow cytometry.
Western Blot: Calpain 2 Antibody (OTI1F4) [NBP2-01814]

Western Blot: Calpain 2 Antibody (OTI1F4) [NBP2-01814]

Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpain 2.
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Applications for Calpain 2 Antibody (OTI1F4)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.75 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain 2

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Alternate Names

Calcium-activated neutral proteinase 2, calpain 2, (m/II) large subunit, calpain 2, large [catalytic] subunit, calpain 2, large subunit, Calpain large polypeptide L2, Calpain M-type, calpain, large polypeptide L2, calpain-2 catalytic subunit, Calpain-2 large subunit, CANP 2, CANPL2CANP2, CANPml, EC 3.4.22, EC 3.4.22.53, FLJ39928, M-calpain, mCANP, millimolar-calpain

Entrez Gene IDs

824 (Human)

Gene Symbol

CAPN2

Additional Calpain 2 Products

Product Documents for Calpain 2 Antibody (OTI1F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain 2 Antibody (OTI1F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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