Calpain 2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92949

Novus Biologicals, part of Bio-Techne
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NBP2-92949-0.02ml
NBP2-92949-0.1ml
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calpain 2 Antibodies »

Product Summary for Calpain 2 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human Calpain 2 (NP_001739.2). SDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 2 Antibody - BSA Free

Knockout Validated: Calpain 2 Antibody - BSA Free [NBP2-92949]

Knockout Validated: Calpain 2 Antibody - BSA Free [NBP2-92949]

Knockout Validated: Calpain 2 Antibody [NBP2-92949] - Analysis of extracts from normal (control) and CAPN2 knockout (KO) 293T cells, using Calpain 2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in

Applications for Calpain 2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain 2

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Alternate Names

Calcium-activated neutral proteinase 2, calpain 2, (m/II) large subunit, calpain 2, large [catalytic] subunit, calpain 2, large subunit, Calpain large polypeptide L2, Calpain M-type, calpain, large polypeptide L2, calpain-2 catalytic subunit, Calpain-2 large subunit, CANP 2, CANPL2CANP2, CANPml, EC 3.4.22, EC 3.4.22.53, FLJ39928, M-calpain, mCANP, millimolar-calpain

Gene Symbol

CAPN2

Additional Calpain 2 Products

Product Documents for Calpain 2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calpain 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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