Calpain 3 Antibody (1B2)
Novus Biologicals, part of Bio-Techne | Catalog # H00000825-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1B2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CAPN3 (AAH03169.1, 210 a.a. ~ 309 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA
Specificity
Reacts with calpain 3, (p94).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Calpain 3 Antibody (1B2)
Immunocytochemistry/ Immunofluorescence: Calpain 3 Antibody (1B2) [H00000825-M01]
Immunocytochemistry/Immunofluorescence: Calpain 3 Antibody (1B2) [H00000825-M01] - Analysis of monoclonal antibody to CAPN3 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Calpain 3 Antibody (1B2) [H00000825-M01] - Detection limit for recombinant GST tagged CAPN3 is 0.3 ng/ml as a capture antibody.
Applications for Calpain 3 Antibody (1B2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calpain 3
Alternate Names
calpain 3, (p94), Calpain L3, Calpain p94, calpain, large polypeptide L3, calpain-3, CANP 3, CANP3MGC4403, CANPL3, EC 3.4.22, EC 3.4.22.54, LGMD2, LGMD2A, MGC10767, MGC11121, MGC14344, Muscle-specific calcium-activated neutral protease 3, muscle-specific calcium-activated neutral protease 3 large subunit, NCL1, nCL-1large [catalytic] subunit, New calpain 1
Entrez Gene IDs
825 (Human)
Gene Symbol
CAPN3
UniProt
Additional Calpain 3 Products
Product Documents for Calpain 3 Antibody (1B2)
Product Specific Notices for Calpain 3 Antibody (1B2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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