Calpain 3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31756
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Calpain 3 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 3. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Porcine (88%), Rat (89%), Bovine (88%), Sheep (87%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calpain 3 Antibody
![Western Blot: Calpain 3 Antibody [NBP1-31756]](https://resources.bio-techne.com/images/products/Calpain-3-Antibody-Western-Blot-NBP1-31756-img0003.jpg "Western Blot: Calpain 3 Antibody [NBP1-31756]")
Western Blot: Calpain 3 Antibody [NBP1-31756]
Western Blot: Calpain 3 Antibody [NBP1-31756] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: Calpain 3 Antibody [NBP1-31756]](https://resources.bio-techne.com/images/products/Calpain-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31756-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Calpain 3 Antibody [NBP1-31756]")
Immunocytochemistry/ Immunofluorescence: Calpain 3 Antibody [NBP1-31756]
Immunocytochemistry/Immunofluorescence: Calpain 3 Antibody [NBP1-31756] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.Applications for Calpain 3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calpain 3
Alternate Names
calpain 3, (p94), Calpain L3, Calpain p94, calpain, large polypeptide L3, calpain-3, CANP 3, CANP3MGC4403, CANPL3, EC 3.4.22, EC 3.4.22.54, LGMD2, LGMD2A, MGC10767, MGC11121, MGC14344, Muscle-specific calcium-activated neutral protease 3, muscle-specific calcium-activated neutral protease 3 large subunit, NCL1, nCL-1large [catalytic] subunit, New calpain 1
Gene Symbol
CAPN3
Additional Calpain 3 Products
Product Documents for Calpain 3 Antibody
Product Specific Notices for Calpain 3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.