Calpain 5 Antibody (HL2432) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25394
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2432
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Calpain 5 Antibody (HL2432) - Azide and BSA Free
Immunogen
Recombinant fragment of human Calpain 5.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calpain 5 Antibody (HL2432) - Azide and BSA Free
Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] -
Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Calpain 5 antibody [HL2432] detects Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calpain 5 stained by Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] -
Western Blot: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] -
Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Calpain 5 antibody [HL2432] detects secreted Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Calpain 5 stained by Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Calpain 5 Antibody (HL2432) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calpain 5
Alternate Names
calpain 5, Calpain htra-3, calpain-5, EC 3.4.22, EC 3.4.22.54, FLJ46245, HTRA3NCL3, nCL-3EC 3.4.22.-, New calpain 3
Gene Symbol
CAPN5
Additional Calpain 5 Products
Product Documents for Calpain 5 Antibody (HL2432) - Azide and BSA Free
Product Specific Notices for Calpain 5 Antibody (HL2432) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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