Calpain 5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92674

Novus Biologicals, part of Bio-Techne
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NBP2-92674-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calpain 5 Antibodies »

Product Summary for Calpain 5 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 361-640 of human CAPN5 (NP_004046.2). GAWTLHEDPRQNRGGGCINHKDTFFQNPQYIFEVKKPEDEVLICIQQRPKRSTRREGKGENLAIGFDIYKVEENRQYRMHSLQHKAASSIYINSRSVFLRTDQPEGRYVIIPTTFEPGHTGEFLLRVFTDVPSNCRELRLDEPPHTCWSSLCGYPQLVTQVHVLGAAGLKDSPTGANSYVIIKCEGDKVRSAVQKGTSTPEYNVKGIFYRKKLSQPITVQVWNHRVLKDEFLGQVHLKADPDNLQALHTLHLRDRNSRQPSNLPGTVAVHILSSTSLMAV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 5 Antibody - BSA Free

Western Blot: Calpain 5 AntibodyBSA Free [NBP2-92674]

Western Blot: Calpain 5 AntibodyBSA Free [NBP2-92674]

Western Blot: Calpain 5 Antibody [NBP2-92674] - Analysis of extracts of various cell lines, using Calpain 5 .Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Calpain 5 Antibody - BSA Free [NBP2-92674]

Immunocytochemistry/ Immunofluorescence: Calpain 5 Antibody - BSA Free [NBP2-92674]

Immunocytochemistry/Immunofluorescence: Calpain 5 Antibody [NBP2-92674] - Analysis of NIH-3T3 cells using Calpain 5 at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for Calpain 5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain 5

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]

Alternate Names

calpain 5, Calpain htra-3, calpain-5, EC 3.4.22, EC 3.4.22.54, FLJ46245, HTRA3NCL3, nCL-3EC 3.4.22.-, New calpain 3

Gene Symbol

CAPN5

Additional Calpain 5 Products

Product Documents for Calpain 5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain 5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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