Calpastatin Antibody (2C5)
Novus Biologicals, part of Bio-Techne | Catalog # H00000831-M03
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2C5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CAST (NP_001741.3, 582 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQ
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
CAST - calpastatin (2C5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Calpastatin Antibody (2C5)
![Western Blot: Calpastatin Antibody (2C5) [H00000831-M03]](https://resources.bio-techne.com/images/products/Calpastatin-Antibody-2C5-Western-Blot-H00000831-M03-img0005.jpg "Western Blot: Calpastatin Antibody (2C5) [H00000831-M03]")
Western Blot: Calpastatin Antibody (2C5) [H00000831-M03]
Western Blot: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of CAST expression in NIH/3T3 (Cat # L018V1).![Immunocytochemistry/ Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03]](https://resources.bio-techne.com/images/products/Calpastatin-Antibody-2C5-Immunocytochemistry-Immunofluorescence-H00000831-M03-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03]")
Immunocytochemistry/ Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03]
Immunocytochemistry/Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of monoclonal antibody to CAST on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Calpastatin Antibody (2C5) [H00000831-M03] - Detection limit for recombinant GST tagged CAST is 0.03 ng/ml as a capture antibody.
Applications for Calpastatin Antibody (2C5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Sandwich ELISA
1:100-1:2000
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calpastatin
Alternate Names
BS-17, Calpain inhibitor, calpastatin, MGC9402, Sperm BS-17 component
Entrez Gene IDs
831 (Human)
Gene Symbol
CAST
Additional Calpastatin Products
Product Documents for Calpastatin Antibody (2C5)
Product Specific Notices for Calpastatin Antibody (2C5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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