Calpastatin Antibody (2C5)

Novus Biologicals, part of Bio-Techne | Catalog # H00000831-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calpastatin Antibodies »

Product Specifications

Immunogen

CAST (NP_001741.3, 582 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

CAST - calpastatin (2C5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Calpastatin Antibody (2C5)

Western Blot: Calpastatin Antibody (2C5) [H00000831-M03]

Western Blot: Calpastatin Antibody (2C5) [H00000831-M03]

Western Blot: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of CAST expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03]

Immunocytochemistry/ Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03]

Immunocytochemistry/Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of monoclonal antibody to CAST on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Calpastatin Antibody (2C5) [H00000831-M03] - Detection limit for recombinant GST tagged CAST is 0.03 ng/ml as a capture antibody.

Applications for Calpastatin Antibody (2C5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpastatin

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length natures of only some have been determined. [provided by RefSeq]

Alternate Names

BS-17, Calpain inhibitor, calpastatin, MGC9402, Sperm BS-17 component

Entrez Gene IDs

831 (Human)

Gene Symbol

CAST

Additional Calpastatin Products

Product Documents for Calpastatin Antibody (2C5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpastatin Antibody (2C5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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