Calpastatin Antibody (CAST/1550)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07397

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CAST/1550

Concentration

0.2 mg/ml

View all Calpastatin Antibodies »

Product Summary for Calpastatin Antibody (CAST/1550)

Immunogen

Recombinant full-length human Calpastatin protein (Uniprot: P20810)

Localization

Cytoplasm and Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08510)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calpastatin Antibody (CAST/1550)

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Tonsil stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).
Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).
Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) [NBP3-07397] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).
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Applications for Calpastatin Antibody (CAST/1550)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calpastatin

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When Calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Alternate Names

BS-17, Calpain inhibitor, calpastatin, MGC9402, Sperm BS-17 component

Gene Symbol

CAST

UniProt

P20810 (Human)

Additional Calpastatin Products

Product Documents for Calpastatin Antibody (CAST/1550)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calpastatin Antibody (CAST/1550)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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