Calpastatin Antibody (OTI1E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01819

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E7

Concentration

0.62 mg/ml

View all Calpastatin Antibodies »

Product Summary for Calpastatin Antibody (OTI1E7)

Immunogen

Full length human recombinant protein of human CAST(NP_001741) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

84.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpastatin Antibody (OTI1E7)

Western Blot: Calpastatin Antibody (OTI1E7) [NBP2-01819]

Western Blot: Calpastatin Antibody (OTI1E7) [NBP2-01819]

Western Blot: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpastatin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpastatin.
Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] Staining of paraffin-embedded Human prostate tissue using anti-Calpastatin mouse monoclonal antibody.
Flow Cytometry: Calpastatin Antibody (OTI1E7) [NBP2-01819]

Flow Cytometry: Calpastatin Antibody (OTI1E7) [NBP2-01819]

Flow Cytometry: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpastatin antibody, and then analyzed by flow cytometry.
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Applications for Calpastatin Antibody (OTI1E7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.62 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpastatin

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].

Alternate Names

BS-17, Calpain inhibitor, calpastatin, MGC9402, Sperm BS-17 component

Entrez Gene IDs

831 (Human)

Gene Symbol

CAST

Additional Calpastatin Products

Product Documents for Calpastatin Antibody (OTI1E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpastatin Antibody (OTI1E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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