Calpastatin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15678

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calpastatin Antibodies »

Product Summary for Calpastatin Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpastatin. The exact sequence is proprietary.

Reactivity Notes

Rabbit (80%), Bovine (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpastatin Antibody

Western Blot: Calpastatin Antibody [NBP2-15678]

Western Blot: Calpastatin Antibody [NBP2-15678]

Western Blot: Calpastatin Antibody [NBP2-15678] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: Calpastatin Antibody [NBP2-15678]

Immunohistochemistry-Paraffin: Calpastatin Antibody [NBP2-15678]

Immunohistochemistry-Paraffin: Calpastatin Antibody [NBP2-15678] - Paraffin-embedded human lung cancer. Calpastatin antibody diluted at 1:500.

Applications for Calpastatin Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpastatin

The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length natures of only some have been determined. [provided by RefSeq]

Alternate Names

BS-17, Calpain inhibitor, calpastatin, MGC9402, Sperm BS-17 component

Gene Symbol

CAST

UniProt

P20810 (Human)

Additional Calpastatin Products

Product Documents for Calpastatin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpastatin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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