Calretinin Antibody (3G9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50042

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G9

Concentration

1 mg/ml

View all Calretinin Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Calretinin expressed in and purified from E. coli. [UniProt# P22676]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calretinin Antibody (3G9)

Western Blot: Calretinin Antibody (3G9) [NBP2-50042]

Western Blot: Calretinin Antibody (3G9) [NBP2-50042]

Western Blot: Calretinin Antibody (3G9) [NBP2-50042] - Analysis of recombinant protein solutions using mouse mAb to calretinin, NBP2-50042, dilution 1:1,000: [1] protein standard, [2] calbindin, [3] calretinin, [4] parvalbumin, [5] secretogogin. This antibody reacts only with calretinin, and does not bind to other related calcium-binding proteins.
Immunohistochemistry Free-Floating: Calretinin Antibody (3G9) [NBP2-50042]

Immunohistochemistry Free-Floating: Calretinin Antibody (3G9) [NBP2-50042]

Immunohistochemistry Free-Floating: Calretinin Antibody (3G9) [NBP2-50042] - Analysis of rat cerebellum section stained with mouse mAb to calretinin, NBP2-50042, dilution 1:500 in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The calretinin antibody labels interneurons in the granule cell layer, while the calbindin antibody stains Purkinje neurons and their dendritic processes in the molecular layer of the cerebellum.

Applications for Calretinin Antibody (3G9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

UniProt

P22676 (Human)

Additional Calretinin Products

Product Documents for Calretinin Antibody (3G9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calretinin Antibody (3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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