Calretinin Antibody (CALB2/2602) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08323

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CALB2/2602

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Calretinin Antibodies »

Product Summary for Calretinin Antibody (CALB2/2602) - Azide and BSA Free

Immunogen

Recombinant human Calretinin protein fragment (around aa23-242) (exact sequence is proprietary) (Uniprot: P22676)

Localization

Cytoplasmic and Nuclear

Specificity

It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.

Marker

Mesothelioma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP3-07365).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calretinin Antibody (CALB2/2602) - Azide and BSA Free

Western Blot: Calretinin Antibody (CALB2/2602)Azide and BSA Free [NBP3-08323]

Western Blot: Calretinin Antibody (CALB2/2602)Azide and BSA Free [NBP3-08323]

Western Blot: Calretinin Antibody (CALB2/2602) [NBP3-08323] - Western Blot Analysis of human Brain tissue lysate using Calretinin Mouse Monoclonal Antibody (CALB2/2602).
Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) - Azide and BSA Free [NBP3-08323]

Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) - Azide and BSA Free [NBP3-08323]

Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) [NBP3-08323] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).
Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) - Azide and BSA Free [NBP3-08323]

Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) - Azide and BSA Free [NBP3-08323]

Immunohistochemistry-Paraffin: Calretinin Antibody (CALB2/2602) [NBP3-08323] - Formalin-fixed, paraffin-embedded human Cerebellum stained with Calretinin Mouse Monoclonal Antibody (CALB2/2602).
View 2 more images

Applications for Calretinin Antibody (CALB2/2602) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

Additional Calretinin Products

Product Documents for Calretinin Antibody (CALB2/2602) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calretinin Antibody (CALB2/2602) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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