Calretinin Antibody (CL12842)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18545

Novus Biologicals, part of Bio-Techne
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NBP3-18545-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12842

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calretinin Antibodies »

Product Summary for Calretinin Antibody (CL12842)

Immunogen

This antibody was developed against a recombinant protein of P22676. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Calretinin Antibody (CL12842)

Western Blot: Calretinin Antibody (CL12842) [NBP3-18545]

Western Blot: Calretinin Antibody (CL12842) [NBP3-18545]

Western Blot: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.
Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545]

Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545]

Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of mouse brain shows strong cytoplasmic positivity in cells in granular layer.
Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545]

Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545]

Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cerebral cortex and liver tissues using NBP3-18545 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.
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Applications for Calretinin Antibody (CL12842)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

Additional Calretinin Products

Product Documents for Calretinin Antibody (CL12842)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calretinin Antibody (CL12842)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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