Calretinin Antibody (JM12-93)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67206

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM12-93

Concentration

1 mg/ml

View all Calretinin Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human Calretinin aa 60-100. (SwissProt: P22676 Human; SwissProt: Q08331 Mouse; SwissProt: P47728 Rat)

Localization

Cuticular plate, cytosol, nucleus, synaptic membrane, dendrite, gap junction, neuron projection, parallel fiber to Purkinje cell synapse, stereocilium, synapse, terminal bouton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calretinin Antibody (JM12-93)

Western Blot: Calretinin Antibody (JM12-93) [NBP2-67206]

Western Blot: Calretinin Antibody (JM12-93) [NBP2-67206]

Western Blot: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of Calretinin on mouse brain tissue lysates with Rabbit anti-Calretinin antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206]

Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206]

Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of paraffin-embedded rat brain tissue using anti-Calretinin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206]

Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206]

Immunohistochemistry-Paraffin: Calretinin Antibody (JM12-93) [NBP2-67206] - Analysis of paraffin-embedded human brain tissue using anti-Calretinin antibody. Counter stained with hematoxylin.
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Applications for Calretinin Antibody (JM12-93)

Application
Recommended Usage

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

Additional Calretinin Products

Product Documents for Calretinin Antibody (JM12-93)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calretinin Antibody (JM12-93)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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