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Calsenilin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00359

Novus Biologicals, part of Bio-Techne
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NBP3-00359-100ul
NBP3-00359-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Calsenilin (Uniprot#: Q9Y2W7-1; Met1-Ile256)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Calsenilin Antibody

Western Blot: Calsenilin Antibody [NBP3-00359]

Western Blot: Calsenilin Antibody [NBP3-00359]

Western Blot: Calsenilin Antibody [NBP3-00359] - Anti-Calsenilin rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse heart Tissue Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 36 kDa
Immunoprecipitation: Calsenilin Antibody [NBP3-00359]

Immunoprecipitation: Calsenilin Antibody [NBP3-00359]

Immunoprecipitation: Calsenilin Antibody [NBP3-00359] - Calsenilin was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg THP-1 Whole Cell Lysate. 0.5 ul anti-Calsenilin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Calsenilin rabbit polyclonal antibody, at 1:350 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 35 kDa

Applications for Calsenilin Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calsenilin

The human prodynorphin gene, which is involved in memory acquisition and pain, is regulated by its downstream regulatory element (DRE). The DRE acts as a location-dependent gene silencer. DREAM is a transcriptional repressor, DRE-antagonist modulator. It is the first known Ca2+ binding protein that binds to DNA and regulates transcription.

Alternate Names

calsenilin, calsenilin, presenilin-binding protein, EF hand transcription factor, CSEN MGC18289, DREAM, DREAMA-type potassium channel modulatory protein 3, KChIP3, KCHIP3 DRE-antagonist modulator, Kv channel interacting protein 3, calsenilin, Kv channel-interacting protein 3, potassium channel interacting protein 3

Gene Symbol

KCNIP3

Additional Calsenilin Products

Product Documents for Calsenilin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Calsenilin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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