Calsequestrin 1 Antibody (5P6U2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15831

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-15831-100ul
NBP3-15831-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5P6U2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calsequestrin 1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_001222.3). EDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calsequestrin 1 Antibody (5P6U2)

Western Blot: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831]

Western Blot: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831]

Western Blot: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831] - Western blot analysis of extracts of Rat heart, using Calsequestrin 1 Rabbit mAb (NBP3-15831) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831]

Immunohistochemistry: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831]

Immunohistochemistry: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831] - Immunofluorescence analysis of Rat skeletal muscle using Calsequestrin 1 antibody (NBP3-15831) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831]

Immunohistochemistry: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831]

Immunohistochemistry: Calsequestrin 1 Antibody (5P6U2) [NBP3-15831] - Immunofluorescence analysis of Mouse skeletal muscle using Calsequestrin 1 antibody (NBP3-15831) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for Calsequestrin 1 Antibody (5P6U2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calsequestrin 1

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

Alternate Names

Calmitin, calmitine, calsequestrin 1 (fast-twitch, skeletal muscle), Calsequestrin, skeletal muscle isoform, calsequestrin-1, CASQ, PDIB1

Gene Symbol

CASQ1

Additional Calsequestrin 1 Products

Product Documents for Calsequestrin 1 Antibody (5P6U2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Calsequestrin 1 Antibody (5P6U2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.