CaM Kinase II alpha Antibody (S09-9E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19589

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S09-9E5

Concentration

0.3 mg/ml

View all CaM Kinase II alpha Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human CaM Kinase II alpha (Uniprot # Q9UQM7)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CaM Kinase II alpha Antibody (S09-9E5)

Western Blot: CaM Kinase II alpha Antibody (S09-9E5) [NBP3-19589]

Western Blot: CaM Kinase II alpha Antibody (S09-9E5) [NBP3-19589]

Western Blot: CaM Kinase II alpha Antibody (S09-9E5) [NBP3-19589] - Western blot detection of CaM Kinase II alpha in Rat Brain, Hela cell lysates using NBP3-19589 (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.

Applications for CaM Kinase II alpha Antibody (S09-9E5)

Application
Recommended Usage

Western Blot

1/1000-1/2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Gene Symbol

CAMK2A

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha Antibody (S09-9E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaM Kinase II alpha Antibody (S09-9E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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