CaM Kinase II Antibody (9P2E2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15256

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15256-100ul
NBP3-15256-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9P2E2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CaM Kinase II Antibodies »

Product Summary for CaM Kinase II Antibody (9P2E2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CaM Kinase II (NP_741960.1). GVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaM Kinase II Antibody (9P2E2)

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded mouse spinal cord using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256]

Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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Applications for CaM Kinase II Antibody (9P2E2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CaM Kinase II

Calmodulin kinase II (CaMKII) is an 8 - 12 subunit multimeric calcium/calmodulin dependent Ser/Thr protein kinase. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes (alpha, beta, gamma, delta), each of which contains a catalytic, autoregulatory, and subunit-association domain.

Long Name

Calcium/Calmodulin-dependent Protein Kinase II

Alternate Names

CAMK2, CAMK2B

Gene Symbol

CAMK2B

Additional CaM Kinase II Products

Product Documents for CaM Kinase II Antibody (9P2E2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaM Kinase II Antibody (9P2E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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