CaM Kinase II [p Thr286] Antibody (JE51-81)

Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752]

Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752] - Western blot analysis of CaM Kinase II on different lysates with Rabbit anti-CaM Kinase II antibody (NBP3-32752) at 1/1,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Mouse brain treated with pp for 1 hour tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 54~73 kDa
Observed band size: 50~60 kDa

Exposure time: 53 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.