CaMKIV Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-287

Novus Biologicals, part of Bio-Techne
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NB300-287
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CaMKIV Antibodies »

Product Summary for CaMKIV Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residues 400 and 425 of human Calcium/Calmodulin-dependent Protein Kinase IV using the numbering given in Swiss-Prot entry Q16566 (GeneID 814).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaMKIV Antibody

Western Blot: CaMKIV Antibody [NB300-287]

Western Blot: CaMKIV Antibody [NB300-287]

Western Blot: CaMKIV Antibody [NB300-287] - A) Detection of CamK4 by Western blot in Jurkat WCE (5, 15, 50 ug) and HeLa WCE (50 ug) using NB300-287 (0.04 ug/ml). B) Immunoprecitation of CamK4 using various CamK4 antibodies (3 ug/mg lysate) and then tested in Western blot on Jurkat WCE (1 mg/IP; 1/4 of IP loaded/lane) using NB300-287 (0.04 ug/ml). Chemiluminescent detection at 30 seconds.

Applications for CaMKIV Antibody

Application
Recommended Usage

Immunoprecipitation

3-5 ug/mg lysate

Western Blot

1:5000-1:15000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CaMKIV

Ca2+/calmodulin dependent protein kinase type 4 (CaMK4, formerly known as CaM kinase Gr) belongs to the serine/threonine protein kinase family, and to the Ca2+/calmodulin dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase. CaMK4 is highly expressed in cell and tissue extracts such as the brain, lymphocytes, spleen, neurons, thymus and testes. This kinase has two isoforms, a catalytically active calmodulin dependent kinase or a testes specific calmodulin binding protein, calspermin, generated by alternative splicing from the same gene. CaMK4 appears to require another kinase (CaMKK) to stimulate it. CaMK4, in a calcium and calmodulin dependent manner, is implicated in phosphorylating transcription factors including the CCAAT enhancer binding protein (C/EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium dependent gene expression. In neurons, CaMK4 is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T cells (NFAT). CaMK4 is encoded, along with calspermin, by the CaMK4 gene. It has been found that in testes, CaMK4 is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

Alternate Names

brain Ca(2+)-calmodulin-dependent protein kinase type IV, brain Ca++-calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase IV, calcium/calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase type IV catalytic chain, CAM kinase- GR, CAM kinase IV, CaM kinase-GR, caMK, CaMK IV, CaMK-GR, EC 2.7.11, EC 2.7.11.17, IV, MGC36771

Entrez Gene IDs

814 (Human)

Gene Symbol

CAMK4

UniProt

Q16566 (Human)

Additional CaMKIV Products

Product Documents for CaMKIV Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaMKIV Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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