CaMKV Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06485
![Western Blot: CaMKV Antibody [NBP3-06485]](https://resources.bio-techne.com/images/products/CaMKV-Antibody-Western-Blot-NBP3-06485-img0001.jpg "Western Blot: CaMKV Antibody [NBP3-06485]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human CaMKV fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CaMKV Antibody
Western Blot: CaMKV Antibody [NBP3-06485]
Western Blot: CaMKV Antibody [NBP3-06485] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa![Immunocytochemistry/ Immunofluorescence: CaMKV Antibody [NBP3-06485]](https://resources.bio-techne.com/images/products/CaMKV-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06485-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CaMKV Antibody [NBP3-06485]")
Immunocytochemistry/ Immunofluorescence: CaMKV Antibody [NBP3-06485]
Immunocytochemistry/Immunofluorescence: CaMKV Antibody [NBP3-06485] - Staining of CAMKV in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CAMKV polyclonal antibody (dilution ratio 1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.![Immunoprecipitation: CaMKV Antibody [NBP3-06485]](https://resources.bio-techne.com/images/products/CaMKV-Antibody-Immunoprecipitation-NBP3-06485-img0003.jpg "Immunoprecipitation: CaMKV Antibody [NBP3-06485]")
Immunoprecipitation: CaMKV Antibody [NBP3-06485]
Immunoprecipitation: CaMKV Antibody [NBP3-06485] - Lane A:0.5 mg HepG2 Whole Cell Lysate1 uL anti-CAMKV rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CAMKV rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 54 kDaObserved band size :54 kDaApplications for CaMKV Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:2500
Immunoprecipitation
0.2-1ul/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CaMKV
Alternate Names
1G5, CaM kinase-like vesicle-associated, caM kinase-like vesicle-associated protein, MGC8407, VACAMKL, vesicle-associated calmodulin-binding protein
Gene Symbol
CAMKV
Additional CaMKV Products
Product Specific Notices for CaMKV Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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