CAMSAP1L1 Antibody

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] -

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] - Loss of non-centrosomal MTs has a more severe impact than their detachment from the Golgi in 3D but not in 2D.(A) Staining for CAMSAP2 (white, green) & Golgi (GM130, red) in HUVECs transfected with indicated siRNA. The plot shows CAMSAP2 enrichment at the Golgi (ratio between the average CAMSAP2 intensity on top of Golgi & in cytoplasm), n = 20 cells per condition. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] -

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] - CAMSAP2 is required for maintaining non-centrosomal MTs & cell migration in ECs.(G) Staining for CAMSAP2 (white/green), gamma-tubulin (red) & DNA (DAPI, blue) in HUVECs. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] -

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] - The centrosome is not essential for angiogenic migration & sprouting.(A) WBs of total extract from HUVECs treated or not treated with centrinone used to quantify the levels of alpha-tubulin & CAMSAP2 protein expression as well as of tubulin post-translational modifications (acetylated, detyrosinated & polyglutamylated tubulin); Ku80 antibody used as loading control, n = 3 independent experiments & n = 4 for CAMSAP2. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] -

Western Blot: CAMSAP1L1 Antibody [NBP1-21402] - CAMSAP2 is required for maintaining non-centrosomal MTs & cell migration in ECs. (E) WBs of extracts of HUVECs treated as in (D) used to quantify the levels of alpha-tubulin & CAMSAP2 expression & the levels of tubulin post-translational modifications (acetylated, detyrosinated & polyglutamylated tubulin); Ku80 antibody used as loading control, n = 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] -

Immunocytochemistry/ Immunofluorescence: CAMSAP1L1 Antibody [NBP1-21402] - CAMSAP2 participates in Golgi polarization during 2D migration & 3D sprouting. (C) Staining for CAMSAP2 (white/green), Golgi (GM130, red), F-actin (phalloidin, white) & DNA (DAPI, blue) in HUVEC spheroid sprouting 14 hr after 3D spheroid embedding. Z-maximum projections of confocal images shown; arrows show polarized Golgi co-localizing with CAMSAP2 stretches & the dashed line indicates the position of spheroid body. Data shown using box plots; Student’s unpaired two-tailed t-test (A), Mann-Whitney U test (B): *p<0.05, ns, no significant difference.10.7554/eLife.33864.028Figure 5—figure supplement 1—source data 1.An Excel sheet with numerical data on the quantification of effect of CAMSAP2 depletion on the polarization of centrosome during migration & the speed, duration & length of Rab6 tracks in 2D migrating ECs represented as plots in Figure 5—figure supplement 1A,B.An Excel sheet with numerical data on the quantification of effect of CAMSAP2 depletion on the polarization of centrosome during migration & the speed, duration & length of Rab6 tracks in 2D migrating ECs represented as plots in Figure 5—figure supplement 1A,B. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/33864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.