Cannabinoid R1/CB1/CNR1 Antibody (PSH02-43)

Western Blot: Cannabinoid R1/CB1/CNR1 Antibody (PSH02-43) [NBP3-32103]

Western Blot: Cannabinoid R1/CB1/CNR1 Antibody (PSH02-43) [NBP3-32103] - Western blot analysis of Cannabinoid R1/CB1/CNR1 on different lysates with Rabbit anti-Cannabinoid R1/CB1/CNR1 antibody (NBP3-32103) at 1/1,000 dilution.

Lane 1: Human brain tissue lysate
Lane 2: Human liver tissue lysate (negative control)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse cerebellum tissue lysate
Lane 5: Mouse liver tissue lysate (negative control)
Lane 6: Rat brain tissue lysate
Lane 7: Rat cerebellum tissue lysate
Lane 8: Rat liver tissue lysate (negative control)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 42 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32103) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cannabinoid R1/CB1/CNR1 Antibody (PSH02-43) [NBP3-32103]

Immunohistochemistry: Cannabinoid R1/CB1/CNR1 Antibody (PSH02-43) [NBP3-32103] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Cannabinoid R1/CB1/CNR1 antibody (NBP3-32103) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32103) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.