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CapG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90214

Novus Biologicals, part of Bio-Techne
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NBP1-90214
NBP1-90214-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CapG Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CapG Antibody

Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214]

Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214]

Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Analysis in human lung and liver tissues. Corresponding CAPG RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214]

Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214]

Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lung, lymph node, skeletal muscle and small intestine using Anti-CAPG antibody (A) NBP1-90214 shows similar protein distribution across tissues to independent antibody NBP1-90215 (B).
Western Blot: CapG Antibody [NBP1-90214]

Western Blot: CapG Antibody [NBP1-90214]

Western Blot: CapG Antibody [NBP1-90214] - Analysis using Anti-CAPG antibody NBP1-90214 (A) shows similar pattern to independent antibody NBP1-90213 (B).

Applications for CapG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CapG

Actin Regulatory Protein encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described.

Long Name

Capping Protein, Gelsolin-like

Alternate Names

AFCP

Gene Symbol

CAPG

Additional CapG Products

Product Documents for CapG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CapG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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