Capicua Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33420

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-33420
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Capicua Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PKRKMRRRSSCSSEPNTPKSAKCEGDIFTFDRTGTEAEDVLGELEYDKVPYSSLRRTLDQRRALVMQLFQDHGFFPSAQATAAFQARYADIFPSKVCLQLKIREVRQKIMQA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Capicua Antibody

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420]

Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues . Corresponding CIC RNA-seq data are presented for the same tissues.
Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CIC antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420]

Western Blot: Capicua Antibody [NBP2-33420] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented
View 6 more images

Applications for Capicua Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Capicua

CIC, a human homolog of Drosophila capicua, encodes a high mobility group box transcription factor and is fused to a double homeodomain gene DUX4 as a result of a recurrent chromosomal translocation. It is involved in epidermal growth factor receptor (EGFR) signaling, developmental patterning, and cell fate. In humans, CIC may be involved in granule cell lineage, cerebellar development, and medulloblastoma tumorigenesis. Recent evidence indicates that CIC is involved in Spinocerebellar Ataxia Type 1 (SCA-1) where it interacts directly with the product of the SCA-1 gene Ataxin-1.

Alternate Names

capicua homolog (Drosophila), KIAA0306capicua (Drosophila) homolog, protein capicua homolog

Gene Symbol

CIC

UniProt

Q96RK0 (Human)

Additional Capicua Products

Product Documents for Capicua Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Capicua Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.