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Carbonic Anhydrase I/CA1 Antibody (1F1)

Novus Biologicals, part of Bio-Techne | Catalog # H00000759-M10

Novus Biologicals, part of Bio-Techne
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H00000759-M10

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1F1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CA1 (AAH27890, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF

Specificity

CA1 (1F1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody (1F1)

Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]

Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]

Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.
Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]

Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]

Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in human lung cancer.
Sandwich ELISA: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Detection limit for recombinant GST tagged CA1 is approximately 0.1ng/ml as a capture antibody.

Applications for Carbonic Anhydrase I/CA1 Antibody (1F1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against tissue lysate for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase I/CA1

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.

Alternate Names

CA1

Entrez Gene IDs

759 (Human)

Gene Symbol

CA1

OMIM

114800 (Human)

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Carbonic Anhydrase I/CA1 Antibody (1F1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody (1F1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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