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Carbonic Anhydrase II/CA2 Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77742

Novus Biologicals, part of Bio-Techne
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NBP1-77742-0.1mg

Key Product Details

Species Reactivity

Validated:

Human, Bovine

Applications

ELISA, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Summary for Carbonic Anhydrase II/CA2 Antibody [Biotin]

Immunogen

Carbonic Anhydrase II/CA2/CA2 [Bovine Erythrocytes] (Uniprot: P00921)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 9336012).

Localization

Cytoplasmic

Specificity

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]

Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody [Biotin]

ELISA: Carbonic Anhydrase II/CA2 Antibody [Biotin] [NBP1-77742] - ELISA results of purified Rabbit Carbonic Anhydrase II/CA2/CA2 antibody [Biotin] Antibody tested against Carbonic Anhydrase II/CA2. Each well was coated in duplicate with 1.0 ug of Carbonic Anhydrase II/CA2. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin HRP conjugate, and TMB substrate.

Applications for Carbonic Anhydrase II/CA2 Antibody [Biotin]

Application
Recommended Usage

ELISA

1:2000-1:10000

Western Blot

1:200-1:1000
Application Notes
This product has been tested against 1.0 ug of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase II/CA2

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

Alternate Names

CA2

Gene Symbol

CA2

UniProt

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Carbonic Anhydrase II/CA2 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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