Carbonic Anhydrase III/CA3 Antibody (006)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89538

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 006

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Carbonic Anhydrase III/CA3 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase III/CA3 (Accession#: NP_005172.1; Met1-Lys260).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Carbonic Anhydrase III/CA3 Antibody (006)

Western Blot: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538]

Western Blot: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538]

Western Blot: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538] - Lane A: HepG2 Whole Cell Lysate Lane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:34 kDa
Immunoprecipitation: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538]

Immunoprecipitation: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538]

Immunoprecipitation: Carbonic Anhydrase III/CA3 Antibody (006) [NBP2-89538] - Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL anti-CA3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CA3 rabbit monoclonal antibody,at 1:250 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 30 kDa Observed band size: 30 kDa

Applications for Carbonic Anhydrase III/CA3 Antibody (006)

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase III/CA3

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.

Alternate Names

CA3

Gene Symbol

CA3

Additional Carbonic Anhydrase III/CA3 Products

Product Documents for Carbonic Anhydrase III/CA3 Antibody (006)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase III/CA3 Antibody (006)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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