Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26744

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CA3/7883

Concentration

0.2 mg/ml

View all Carbonic Anhydrase III/CA3 Antibodies »

Product Summary for Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

Immunogen

Recombinant fragment of human Carbonic Anhydrase III/CA3 protein (exact sequence is proprietary)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

Immunohistochemistry-Paraffin: Carbonic Anhydrase III/CA3 Antibody (CA3/7883) [NBP3-26744 ] -

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Carbonic Anhydrase III/CA3 Antibody (CA3/7883). Inset: PBS instead of primary antibody; secondary only negative control.
Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

Western Blot: Carbonic Anhydrase III/CA3 Antibody (CA3/7883) [NBP3-26744 ] -

Western blot analysis of RH-30 cell lysate using Carbonic Anhydrase III/CA3 Antibody (CA3/7883).

Applications for Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml for 30 minutes at RT

Western Blot

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Carbonic Anhydrase III/CA3

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encode carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.

Alternate Names

CA3

Gene Symbol

CA3

Additional Carbonic Anhydrase III/CA3 Products

Product Documents for Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase III/CA3 Antibody (CA3/7883)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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