Carbonic Anhydrase IV/CA4 Antibody (039)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89518

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 039

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Carbonic Anhydrase IV/CA4 Antibodies »

Product Summary for Carbonic Anhydrase IV/CA4 Antibody (039)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IV/CA4 (Accession#: NP_000708.1; Met1-Lys283).

Specificity

No cross-reactivity in ELISA with: Human CA2Human CA3Human CA5AHuman CA5BHuman CA8Human CA9Human CA10Human CA12Human CA13Human CA14

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Carbonic Anhydrase IV/CA4 Antibody (039)

Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518]

Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518]

Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to renal tubules.
Flow Cytometry: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518]

Flow Cytometry: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518]

Flow Cytometry: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Human primary renal tubular epithelial cells stained with carbonic Anhydrase IV/CA4 antibody conjugated to Alexa Fluor 488 (NBP2-89518AF488). Cells were immediately fixed with 4% PFA after detachment from culture plates and prior to staining. Final Ab concentration was 1:50. Image from verified customer review.
Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518]

Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518]

Immunohistochemistry-Paraffin: Carbonic Anhydrase IV/CA4 Antibody (039) [NBP2-89518] - Staining of human CA4 in human lung with rabbit monoclonal antibody (1:200). Positive staining was localized to pulmonary capillaries.

Applications for Carbonic Anhydrase IV/CA4 Antibody (039)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

Reported from a verified customer review

Immunohistochemistry-Paraffin

1:100-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase IV/CA4

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq]

Alternate Names

CA4, RP17

Gene Symbol

CA4

Additional Carbonic Anhydrase IV/CA4 Products

Product Documents for Carbonic Anhydrase IV/CA4 Antibody (039)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase IV/CA4 Antibody (039)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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