Carbonic Anhydrase VIII/CA8 Antibody (005)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90710
Recombinant Monoclonal Antibody
![Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-VIII-CA8-Antibody-005-Western-Blot-NBP2-90710-img0003.jpg "Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 005
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Carbonic Anhydrase VIII/CA8 Antibody (005)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carbonic Anhydrase VIII/CA8 (Uniprot#: P28651; Met1-Gln291).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Carbonic Anhydrase VIII/CA8 Antibody (005)
Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]
Western Blot: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Anti-Carbonic Anhydrase VIII/CA8 rabbit monoclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:36 kDa![Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-VIII-CA8-Antibody-005-Immunoprecipitation-NBP2-90710-img0002.jpg "Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]")
Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710]
Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody (005) [NBP2-90710] - Mouse CA8 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CA8 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CA8 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 37 kDaApplications for Carbonic Anhydrase VIII/CA8 Antibody (005)
Application
Recommended Usage
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase VIII/CA8
Alternate Names
CA8, CALS, CARP
Gene Symbol
CA8
Additional Carbonic Anhydrase VIII/CA8 Products
Product Documents for Carbonic Anhydrase VIII/CA8 Antibody (005)
Product Specific Notices for Carbonic Anhydrase VIII/CA8 Antibody (005)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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