Carbonic Anhydrase XIV/CA14 Antibody (515)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89510

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative), Rat (Negative)

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 515

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Carbonic Anhydrase XIV/CA14 Antibodies »

Product Summary for Carbonic Anhydrase XIV/CA14 Antibody (515)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase XIV/CA14 (Accession#: NP_036245.1; Met 1-Met 290).

Specificity

No cross-reactivity in ELISA with: Human CARPXHuman CA12Mouse CA14Mouse, Rat

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Carbonic Anhydrase XIV/CA14 Antibody (515)

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510]

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510]

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XIV/CA14 Antibody (515) [NBP2-89510] - Staining of human CA14 in human liver with rabbit monoclonal antibody (1:200). Positive staining was localized to membrane of hepatocyte.

Applications for Carbonic Anhydrase XIV/CA14 Antibody (515)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry-Paraffin

1:100-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XIV/CA14

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq]

Alternate Names

CA14

Gene Symbol

CA14

Additional Carbonic Anhydrase XIV/CA14 Products

Product Documents for Carbonic Anhydrase XIV/CA14 Antibody (515)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase XIV/CA14 Antibody (515)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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