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Carboxylesterase 1/CES1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44236

Novus Biologicals, part of Bio-Techne
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NBP2-44236

Key Product Details

Species Reactivity

Validated:

Porcine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Summary for Carboxylesterase 1/CES1 Antibody

Immunogen

Carboxylesterase 3/CES3/Carboxylesterase 3/CES3/Esterase 31 31 [Porcine Liver] (Uniprot: Q29550)

Reactivity Notes

Cross reactivity against Carboxylesterase 1/CES1 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for Carboxylesterase 1/CES1 Antibody

Application
Recommended Usage

ELISA

1:10000-1:50000

Western Blot

1:1000-1:5000
Application Notes
This product is suitable for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Carboxylesterase 1/CES1

Carboxylesterase 1 hydrolyzes ester- and amide bonds of substrates ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. It plays a major role in the pharmacokinetic behavior of many therapeutic agents containing an ester. Through de-esterification, it participates in the detoxification of drugs such as cocaine and heroin in serum and liver.

Alternate Names

ACAT, CES1, Egasyn, HMSE1, REH, SES1, TGH

Gene Symbol

CES1

Additional Carboxylesterase 1/CES1 Products

Product Documents for Carboxylesterase 1/CES1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carboxylesterase 1/CES1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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