CARD11/CARMA1 Antibody (8N4C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16849

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16849-100ul
NBP3-16849-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8N4C9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CARD11/CARMA1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1055-1154 of human CARD11/CARMA1 (Q9BXL7). EAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTIWVDEDQL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARD11/CARMA1 Antibody (8N4C9)

Western Blot: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849]

Western Blot: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849]

Western Blot: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849] - Western blot analysis of extracts of various cell lines, using CARD11/CARMA1 Rabbit mAb (NBP3-16849) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849]

Immunohistochemistry: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849]

Immunohistochemistry: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849] - Immunofluorescence analysis of mouse spleen using CARD11/CARMA1 Rabbit mAb (NBP3-16849) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849]

Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849]

Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody (8N4C9) [NBP3-16849] - Immunohistochemistry of paraffin-embedded rat spleen using CARD11/CARMA1 Rabbit mAb (NBP3-16849) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for CARD11/CARMA1 Antibody (8N4C9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CARD11/CARMA1

CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.

Long Name

Caspase Recruitment Domain-containing Protein 11

Alternate Names

BIMP3, CARMA1

Gene Symbol

CARD11

Additional CARD11/CARMA1 Products

Product Documents for CARD11/CARMA1 Antibody (8N4C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CARD11/CARMA1 Antibody (8N4C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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