CARD12 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78980

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.09 mg/ml

View all CARD12 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human CARD12 protein (between residues 950-1015)

Localization

Cytoplasm. Note: Cytoplasmic filaments.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CARD12 Antibody - BSA Free [NBP1-78980]

Immunocytochemistry/ Immunofluorescence: CARD12 Antibody - BSA Free [NBP1-78980]

Immunocytochemistry/Immunofluorescence: CARD12 Antibody [NBP1-78980] - Tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: CARD12 Antibody - BSA Free [NBP1-78980]

Immunohistochemistry: CARD12 Antibody - BSA Free [NBP1-78980]

Immunohistochemistry: CARD12 Antibody [NBP1-78980] - Analysis of CARD12 in mouse lung using DAB with hematoxylin counterstain.

Applications for CARD12 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:200
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.09 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD12

CARD12, also known as CLAN, IPAF and CLAN1 is a member of the CED4 family of proteins. The CED4/Apaf-1 proteins are critical regulators of apoptosis and NF-kappaB signaling pathways, and CARD12 is no exception. CARD12 has been implicated in coordination of downstream activation of both apoptotic and inflammatory signaling pathways (PMID: 11374873). Additionally, CARD12 has shown to coprecipitate with caspase 1, an enzyme that participates in both the signaling of apoptosis and cytokine processing, further demonstrating its integral role in programmed cell death (PMID: 11374873). As part of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) proteins, CARD12 is known to be an important regulator of caspase-1 activation via an ATP/dATP nucleotide-binding pocket (PMID: 15882992). CARD12 has four known isoforms. Isoform 1 is highly expressed in lung, followed by leukocytes (especially monocytes), lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Although it is expressed ubiquitously, Isoform 2 is more prominent at the highest levels in lung and spleen. Isoform 4 can only been found in the brain.

Alternate Names

CARD12leucine rich repeat and CARD domaincontaining 4, caspase recruitment domain family, member 12, Caspase recruitment domain-containing protein 12, Clan protein, CLAN1ipaf, CLANCARD, LRR, and NACHT-containing protein, CLR2.1, Ice protease-activating factor, Ipaf, NLR family, CARD domain containing 4

Entrez Gene IDs

58484 (Human)

Gene Symbol

NLRC4

UniProt

Q9NPP4 (Human)

Additional CARD12 Products

Product Documents for CARD12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CARD12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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