CARD14 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92873

Novus Biologicals, part of Bio-Techne
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NBP2-92873-0.02ml
NBP2-92873-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CARD14 Antibodies »

Product Summary for CARD14 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human CARD14 (NP_001244899.1). SLSLHYSNALQEKELAASRCRSLQEELYLLKQELQRANMVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFSLAEKDILEQSLDEARGSRQELVERIHSLRERAVAAERQREQYWEEKEQTLLQFQKSKMACQLYREKVNALQAQVCELQKERDQAYSARDSAQREISQSLVEKDSLRRQVFELTDQVCELRTQLRQLQAEPPGVLKQEARTREPCPREKQRLVRMHAICPRDDSDCSLVSSTESQLLSDLSATSSRELVDSFRSSSPAPPSQQSLYKRVAEDFGEEPWSFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD14 Antibody - Azide and BSA Free

Western Blot: CARD14 AntibodyAzide and BSA Free [NBP2-92873]

Western Blot: CARD14 AntibodyAzide and BSA Free [NBP2-92873]

Western Blot: CARD14 Antibody [NBP2-92873] - Analysis of extracts of various cell lines, using CARD14 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Applications for CARD14 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CARD14

The protein encoded by the CARD14 gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class ofproteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions ofthe plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying acharacteristic caspase-associated recruitment domain (CARD). This protein shares a similar domain structure withCARD11 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein knownto function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this proteinactivated NF-kappaB and induced the phosphorylation of BCL10. Two alternatively spliced variants of this gene encodingdistinct isoforms have been reported. (provided by RefSeq)

Alternate Names

bcl10-interacting maguk protein 2, BIMP2, CARD-containing MAGUK protein 2, card-maguk protein 2, Carma 2, CARMA2CARD-containing MAGUK 2 protein, caspase recruitment domain family, member 14, caspase recruitment domain-containing protein 14

Gene Symbol

CARD14

Additional CARD14 Products

Product Documents for CARD14 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CARD14 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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