CARD9 Antibody (PSH0-41)

Western Blot: CARD9 Antibody (PSH0-41) [NBP3-32106]

Western Blot: CARD9 Antibody (PSH0-41) [NBP3-32106] - Western blot analysis of CARD9 on different lysates with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/1,000 dilution.

Lane 1: HL-60 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 62.2 kDa
Observed band size: 70 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: CARD9 Antibody (PSH0-41) [NBP3-32106]

Immunocytochemistry/ Immunofluorescence: CARD9 Antibody (PSH0-41) [NBP3-32106] - Immunocytochemistry analysis of THP-1 cells labeling CARD9 with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: CARD9 Antibody (PSH0-41) [NBP3-32106]

Flow Cytometry: CARD9 Antibody (PSH0-41) [NBP3-32106] - Flow cytometric analysis of THP-1 cells labeling CARD9.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32106, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).