CARKL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91151

Novus Biologicals, part of Bio-Techne
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NBP1-91151
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CARKL Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARKL Antibody

Western Blot: CARKL Antibody [NBP1-91151]

Western Blot: CARKL Antibody [NBP1-91151]

Western Blot: CARKL Antibody [NBP1-91151] - Analysis in control (vector only transfected HEK293T lysate) and SHPK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CARKL Antibody [NBP1-91151]

Immunohistochemistry-Paraffin: CARKL Antibody [NBP1-91151]

Immunohistochemistry-Paraffin: CARKL Antibody [NBP1-91151] - Staining in human kidney and pancreas tissues using anti-SHPK antibody. Corresponding SHPK RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CARKL Antibody [NBP1-91151]

Immunohistochemistry-Paraffin: CARKL Antibody [NBP1-91151]

Immunohistochemistry-Paraffin: CARKL Antibody [NBP1-91151] - Staining of human kidney shows high expression.
View 1 more images

Applications for CARKL Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARKL

CARKL is encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq]

Alternate Names

carbohydrate kinase-like, Carbohydrate kinase-like protein, CARKLEC 2.7.1.14, sedoheptulokinase, SHKFLJ32478

Gene Symbol

SHPK

Additional CARKL Products

Product Documents for CARKL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CARKL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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