CARKL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61224

Novus Biologicals, part of Bio-Techne
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NBP2-61224
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CARKL Antibodies »

Product Specifications

Immunogen

100% Sequence Identity Orangutan between 250 and 300

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Western blot of immunoprecipitates performed using ReliaBLOT Reagents and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Scientific Data Images for CARKL Antibody

Immunoprecipitation: CARKL Antibody [NBP2-61224]

Immunoprecipitation: CARKL Antibody [NBP2-61224]

Immunoprecipitation: CARKL Antibody [NBP2-61224] - Detection of Human SHPK by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHPK antibody NBP2-61224 used for IP at 20 ul per reaction. SHPK was also immunoprecipitated by rabbit anti-SHPK antibody NBP2-61223. For blotting immunoprecipitated SHPK, NBP2-61224 was used at 1:100. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for CARKL Antibody

Application
Recommended Usage

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Background: CARKL

CARKL is encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq]

Alternate Names

carbohydrate kinase-like, Carbohydrate kinase-like protein, CARKLEC 2.7.1.14, sedoheptulokinase, SHKFLJ32478

Gene Symbol

SHPK

Additional CARKL Products

Product Documents for CARKL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CARKL Antibody

Western blot of immunoprecipitates performed using ReliaBLOT Reagents and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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