CARS Antibody (7V0X1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15264

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15264-20ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7V0X1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CARS Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 650-748 of human CARS (P49589). EREEKRRVEEEKRKKKEEAARRKQEQEAAKLAKMKIPPSEMFLSETDKYSKFDENGLPTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYLQMAQNGSFQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARS Antibody (7V0X1)

Western Blot: CARS Antibody (7V0X1) [NBP3-15264]

Western Blot: CARS Antibody (7V0X1) [NBP3-15264]

Western Blot: CARS Antibody (7V0X1) [NBP3-15264] - Western blot analysis of extracts of various cell lines, using CARS antibody (NBP3-15264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry-Paraffin: CARS Antibody (7V0X1) [NBP3-15264]

Immunohistochemistry-Paraffin: CARS Antibody (7V0X1) [NBP3-15264]

Immunohistochemistry-Paraffin: CARS Antibody (7V0X1) [NBP3-15264] - Immunohistochemistry of paraffin-embedded rat brain using CARS Rabbit mAb (NBP3-15264) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for CARS Antibody (7V0X1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CARS

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

CARS1, CYSRS, cysteine translase, cysteine tRNA ligase 1, cytoplasmic, Cysteine--tRNA ligase, cysteinyl-tRNA synthetase, cysteinyl-tRNA synthetase, cytoplasmic, EC 6.1.1, EC 6.1.1.16, MGC:11246

Gene Symbol

CARS1

Additional CARS Products

Product Documents for CARS Antibody (7V0X1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CARS Antibody (7V0X1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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