CARS Antibody (CL2302)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36755

Novus Biologicals, part of Bio-Techne
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NBP2-36755
NBP2-36755-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2302

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CARS Antibodies »

Product Summary for CARS Antibody (CL2302)

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE

Epitope

RPNQALLENIALYLT

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (86%), Rat (86%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CARS Antibody (CL2302)

Western Blot: CARS Antibody (CL2302) [NBP2-36755]

Western Blot: CARS Antibody (CL2302) [NBP2-36755]

Western Blot: CARS Antibody (CL2302) [NBP2-36755] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos
Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755]

Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755]

Immunohistochemistry: CARS Antibody (CL2302) [NBP2-36755] - Staining of human skeletal muscle shows absence of immunoreacitivity.
Western Blot: CARS Antibody (CL2302) [NBP2-36755]

Western Blot: CARS Antibody (CL2302) [NBP2-36755]

Western Blot: CARS Antibody (CL2302) [NBP2-36755] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
View 4 more images

Applications for CARS Antibody (CL2302)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARS

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

CARS1, CYSRS, cysteine translase, cysteine tRNA ligase 1, cytoplasmic, Cysteine--tRNA ligase, cysteinyl-tRNA synthetase, cysteinyl-tRNA synthetase, cytoplasmic, EC 6.1.1, EC 6.1.1.16, MGC:11246

Gene Symbol

CARS1

UniProt

A8MVQ3 (Human)

Additional CARS Products

Product Documents for CARS Antibody (CL2302)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CARS Antibody (CL2302)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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