CASC5 Antibody (HL2158) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25397
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2158
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for CASC5 Antibody (HL2158) - Azide and BSA Free
Immunogen
Synthtic peptide of human CASC5
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CASC5 Antibody (HL2158) - Azide and BSA Free
Western Blot: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] -
Western Blot: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] - A431 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CASC5 antibody [HL2158] (NBP3-25397) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/Immunofluorescence: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] -
Immunocytochemistry/Immunofluorescence: CASC5 Antibody (HL2158) - Azide and BSA Free [NBP3-25397] - CASC5 antibody [HL2158] detects CASC5 protein at kinetochore by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CASC5 stained by CASC5 antibody [HL2158] (NBP3-25397) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .Applications for CASC5 Antibody (HL2158) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CASC5
Alternate Names
AF15Q14, Blinkin, blinkin, bub-linking kinetochore protein, Bub-linking kinetochore protein, cancer susceptibility candidate 5, Cancer susceptibility candidate gene 5 protein, Cancer/testis antigen 29, CT29KIAA1570, D40, hKNL-1, hSpc105, kinetochore null 1 homolog, Kinetochore-null protein 1, KNL1, KNL1ALL1-fused gene from chromosome 15q14 protein, protein CASC5, Protein D40/AF15q14
Gene Symbol
KNL1
Additional CASC5 Products
Product Documents for CASC5 Antibody (HL2158) - Azide and BSA Free
Product Specific Notices for CASC5 Antibody (HL2158) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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