Casein Kinase 1 epsilon Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92968

Novus Biologicals, part of Bio-Techne
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NBP2-92968-0.02ml
NBP2-92968-0.1ml
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Casein Kinase 1 epsilon Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human Casein Kinase 1 epsilon (NP_689407.1). EFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Casein Kinase 1 epsilon Antibody - BSA Free

Western Blot: Casein Kinase 1 epsilon AntibodyBSA Free [NBP2-92968]

Western Blot: Casein Kinase 1 epsilon AntibodyBSA Free [NBP2-92968]

Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968]

Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968]

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of HeLa cells using Casein Kinase 1 epsilon . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968]

Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968]

Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] Casein Kinase 1 epsilon Rabbit pAb (NBP2-92968) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for Casein Kinase 1 epsilon Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Casein Kinase 1 epsilon

CKI epsilon is a member of the casein kinase 1 (CK1) serine/threonine kinase family of proteins. In mammals, there are seven distinct genes that represent the CK1 family: alpha, beta, gamma-1, gamma-2, gamma-3, delta, and epsilon. CKI epsilon phosphorylates a large number of proteins and is involved in pathways involving Wnt and AKT. CKI epsilon has been found to bind and phosphorylate the circadian protein, Period, and the Wnt signaling protein, Dsh. In the Wnt pathway, CKI epsilon activity is required for the full activation of Dsh and downstream signaling effects.

Alternate Names

CSNK1E

Gene Symbol

CSNK1E

Additional Casein Kinase 1 epsilon Products

Product Documents for Casein Kinase 1 epsilon Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Casein Kinase 1 epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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