Casein Kinase 2 beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06515

Novus Biologicals, part of Bio-Techne
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NBP1-06515
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Casein Kinase 2 beta Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 165 and 215 of human casein kinase 2, beta using the numbering given in entry NP_001311.3 (GeneID 1460).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-1567, NB100-97835, NB200-171

Scientific Data Images for Casein Kinase 2 beta Antibody

Western Blot: Casein Kinase 2 beta Antibody [NBP1-06515]

Western Blot: Casein Kinase 2 beta Antibody [NBP1-06515]

Western Blot: Casein Kinase 2 beta Antibody [NBP1-06515] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CKII beta antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Applications for Casein Kinase 2 beta Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Casein Kinase 2 beta

This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Alternate Names

CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin

Entrez Gene IDs

1460 (Human); 13001 (Mouse)

Gene Symbol

CSNK2B

UniProt

NP_001311.3 (Human)

Additional Casein Kinase 2 beta Products

Product Documents for Casein Kinase 2 beta Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Casein Kinase 2 beta Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-1567, NB100-97835, NB200-171

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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