Caspase-12 Antibody (14F7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1432

Novus Biologicals, part of Bio-Techne
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NB600-1432
NB600-1432-0.025ml
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 14F7

Format

BSA Free

Concentration

2.0 mg/ml

View all Caspase-12 Antibodies »

Product Specifications

Immunogen

Recombinant fragment: GPQKICTPSS PSESKRKVEDD EMEVNAGLAH ESHLM LTAPH GLQSSEVQDTL KLCPRDQFCK IKTERAKEIY PVMEKEGRTRL ALIICNKKFDY LFDRDNADTDI LNMQELLENLG YSVVLKENLTA QEMETELMQFA GRPEHQSSDST FLVFMSHGIL EGICGVKHRNK KPDVLHDDTIF KIFNNSNCRSL RNKPKILIMQA CRGRYNGTIWV STNKGIATAD, corresponding to amino acids 95-318 of Mouse Caspase 12.

Localization

Endoplasmic reticulum

Specificity

This antibody recognizes the full length and possibly the truncated p20 subunit of Caspase 12.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-12 Antibody (14F7) - BSA Free

Western Blot: Caspase-12 Antibody (14F7)BSA Free [NB600-1432]

Western Blot: Caspase-12 Antibody (14F7)BSA Free [NB600-1432]

Western Blot: Caspase-12 Antibody (14F7) - BSA Free [NB600-1432] - NRK Rat kidney cell extract was separated on SDS-PAGE and probed with Monoclonal (RAT) AB TO CASPASE-12 CL:14F7. The antibody was developed with HRP APA Rat IgG and an ECL substrate. Antibody concentration 1ug/mL.

Applications for Caspase-12 Antibody (14F7) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1-2 ug/ml
Application Notes
Immunocytochemistry: 4% paraformaldehyde-fixed, paraffin-embedded). Predicted molecular weight: 50 kDa. Antibody may also work in Immunohistochemistry and immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-12

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene, CASP12, is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. (provided by RefSeq)

Alternate Names

CASP12, Caspase12

Entrez Gene IDs

12364 (Mouse); 156117 (Rat)

Gene Symbol

CASP12

Additional Caspase-12 Products

Product Documents for Caspase-12 Antibody (14F7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-12 Antibody (14F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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