Catalase Antibody (7D2Y4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15357

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15357-100ul
NBP3-15357-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7D2Y4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Catalase Antibodies »

Product Summary for Catalase Antibody (7D2Y4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Catalase (P04040). MADSRDPASDQMQHWKEQRAAQKADVLTTGAGNPVGDKLNVITVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITKYSKAKVF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Catalase Antibody (7D2Y4)

Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357]

Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357]

Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357] - Western blot analysis of extracts of various cell lines, using Catalase Rabbit mAb (NBP3-15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Catalase Antibody (7D2Y4) [NBP3-15357]

Immunohistochemistry-Paraffin: Catalase Antibody (7D2Y4) [NBP3-15357]

Immunohistochemistry-Paraffin: Catalase Antibody (7D2Y4) [NBP3-15357] - Immunohistochemistry of paraffin-embedded Mouse kidney using Catalase antibody (NBP3-15357) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Catalase Antibody (7D2Y4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Catalase

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Alternate Names

Cas1, CAT, Cs-1

Gene Symbol

CAT

Additional Catalase Products

Product Documents for Catalase Antibody (7D2Y4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Catalase Antibody (7D2Y4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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