Catalase Antibody (HPYL/7170) - (H. pylori)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13821

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

H. pylori

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # HPYL/7170

Concentration

0.2 mg/ml

View all Catalase Antibodies »

Product Summary for Catalase Antibody (HPYL/7170) - (H. pylori)

Immunogen

Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary)

Reactivity Notes

Helicobacter pylori

Localization

Cell surface, Cytoplasm

Specificity

The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy.H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14079)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Catalase Antibody (HPYL/7170) - (H. pylori)

Immunohistochemistry-Paraffin: Catalase Antibody (HPYL/7170) - (H. pylori) [NBP3-13821]

Immunohistochemistry-Paraffin: Catalase Antibody (HPYL/7170) - (H. pylori) [NBP3-13821]

Immunohistochemistry-Paraffin: Catalase Antibody (HPYL/7170) - (H. pylori) [NBP3-13821] - Formalin-fixed, paraffin-embedded human H. pylori-infected stomach stained with Catalase antibody (HPYL/7170) - (H. pylori).

Applications for Catalase Antibody (HPYL/7170) - (H. pylori)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Catalase

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Alternate Names

Cas1, CAT, Cs-1

Gene Symbol

CAT

Additional Catalase Products

Product Documents for Catalase Antibody (HPYL/7170) - (H. pylori)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Catalase Antibody (HPYL/7170) - (H. pylori)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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