Cathepsin E Antibody

Western Blot: Cathepsin E Antibody [NB400-152]

Western Blot: Cathepsin E Antibody [NB400-152] - Analysis of Cathepsin E on mouse stomach lysate.

Western Blot: Cathepsin E Antibody [NB400-152] -

Western Blot: Cathepsin E Antibody [NB400-152] - The expression & methylation status of Ctse gene in CD4+ T cells isolated from MRL/lpr lupus-prone (MRL) & C57BL/6 (B6) mice. (A) mRNA expression of Ctse in CD4+, CD8+, B cells & macrophages in B6 & MRL mice. ***p < 0.001. (B) Western blot analyses of CTSE in CD4+, CD8+, B cells & macrophages in B6, MRL & mice. (C) Methylated region expanding 583 bp in Ctse intron 1 in B6 mice demonstrated by UCSC Genome Browser. In MRL mice, 583 bp region is hypomethylated. Duplicate reads are indicated by arrows. (D) Nucleotide sequence within 583 bp flanking region of Ctse intron 1 in B6 mice. Among 13 CpGs (red & underlined), 11th CpG (boxed) includes mCGCG binding motif for Kaiso. After genomic DNAs were bisulphite converted, amplified by PCR using Primer F1 & R1, subcloned, & isolated clones sequenced. 98.6% of CpGs (71 out of 72 colonies) in B6 mice & 76.9% CpGs (50 of 65) in MRL mice were methylated. (E) The structure of Kaiso, SMRT (Silencing Mediator of Retinoic acid & Thyroid hormone receptors)/N-CoR (Nuclear hormone receptor Co-Repressor), & HDAC3 (Histone deacetylase 3) complex. BTB (for BR-C, ttk & bab), POZ (for Pox virus & Zinc finger) & C2H2 zinc-finger domains are shown. (F) DNA sequencing of bisulphite converted DNAs in Ctse intron 1 in B6 & MRL mice. Percentage of methylation of 11th CpG is shown (n = 5). ***p < 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30816218), licensed under a CC-BY license. Not internally tested by Novus Biologicals.